The Journal of Oral Laser Applications
J Oral Laser Applications 10 (2010), No. 4     14. June 2011
J Oral Laser Applications 4 (2004), No. 3  (25.10.2004)

Page 167-170

In Vitro Stimulatory Effect of Diode Laser on the Secretion of Tissue Inhibitor of Matrix Metalloproteinases 1 (TIMP-1) in Human Gingival Fibroblasts: A Preliminary Report
Patruta, Sanda/Franz, Alexander/Beer, Franziska/Goharkhay, Kawe/Sperr, Wolfgang/Moritz, Andreas
Purpose: A key homeostatic mechanism of gingival fibroblasts in tissue repair is maintained through their production of tissue inhibitors of matrix metalloproteinases (TIMP). The purpose of this investigation was to elucidate the influence of diode laser irradiation on the in vitro production of TIMP-1 by human gingival fibroblasts.
Materials and Methods: A human gingival fibroblast cell line (HGFB) was irradiated with a diode laser in pulsed mode at 0.5, 1, and 1.5 W for 5 s five times each with 5-s pauses in between. On the same plates in different wells, cells were then irradiated using continuous wave diode laser at 0.5 W for 5 s five consecutive times, with 5-s pauses in between. The cells were incubated overnight (18 h), and in the supernatants the concentration of free TIMP-1 was measured using a TIPM-1 ELISA.
Results: At all settings, diode laser irradiation significantly increased in vitro TIMP-1 secretion of human gingival fibroblasts (p <= 0.001), measured after 18 hours of incubation. The best results were obtained using continuous wave irradiation with 0.5 W for 5 s applied five consecutive times with 5-s intervals in between. TIMP-1 secretion increased by 42.2% compared to the control.
Conclusion: Our findings show that diode laser irradiation of human gingival fibroblasts signficantly increases TIMP-1 secretion compared to control values. This could contribute to periodontal tissue healing.

Keywords: human gingival fibroblast, matrix metalloproteinases, diode laser